BIOS 1700 Lecture Notes - Lecture 13: Streptococcus, Ribonuclease, Fruit Preserves
6 Dec 2016
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How do we demonstrate that an enzyme can bind to a substrate: -galactosidase experiment. A container is separated into two compartments by a selectively permeable membrane. Part 1: radioactively labeled -galactosidase (s) is added to compartment 1 and the movement of s is followed by measuring the radioactivity levels in the two compartments. Over time, the level of radioactivity is the same in the two compartments. Part 2: radioactively labeled substrate is added to compartment 1 and the enzyme (e) is added to compartment 2. The movement of s is followed by measuring the level of radioactivity in the two compartments. Over time, the level of radioactivity is greater in compartment 2 than in. Conclusion: if the substrate diffuses from compartment to compartment 2, forms a couple with the enzyme and then is not released because the enzyme cannot catalyze the conversion of substrate to product. Reversible inhibition: fake substrate can be released, two types.
