BIOS 4630 Lecture Notes - Lecture 9: Viscosity, Phase Transition, Thylakoid
23 Feb 2017
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Dynamic properties of membranes (frye and edidin 1970: fluorescently-tagged antibodies, used on mouse cells- red wavelength, human cells blue wavelength, applied virus to induce fusion of the cells heterokaryon (forming a hybrid of mouse and human proteins) At 15 degrees there was a lot less mixing and it took a lot longer. Promotes transition from trans to gauche and gauche to trans: energy or heat content increases the speed at which these isomerizations take place. Increase temperature increases potential for transition from trans to gauche. More gauche isomers at high temperature at trans allowing increase in membrane fluidity: trans/gauche is more rapid than lateral diffusion which is more rapid than transverse or flip flop diffusion. Fluorescence recovery after photobleaching (frap: uses a fluorescent tag for either polar lipids or proteins, the fluorescent tag distributes itself homogenously throughout cell surface. After a period of time you monitor the recovery of the bleached area.
