CHE 107 Lecture Notes - Lecture 45: Polymerase Chain Reaction, Electropherogram, Amelogenin
11 Nov 2018
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Polymerase chain reaction (pcr): small pieces of dna called short tandem repeats (str). Q: how does the amplification step actually work: single strand of dna is split into 2 strands, bases are added to make each strands complete, repeated about 32 times, like a copy machine. Electropherogram: end result, which tells us the dna profile of the sample: each dna peak represents an allele, under each peak is allele # & length of dna allele (in base pairs). Amelogenin is sex-determining locus: dna statistics: Population frequency calculation: dna profiles have been obtained for ppl of different races all over the world, profiles are put into a database where frequency of different alleles at all of the loci analyzed is determined. Ex: how often is the 16 allele at d3s1358 seen in the caucasian database?, asian database?, hispanic database?, etc. Frequencies are multiplied together to get a total frequency/population frequency.
