Detection of specific dna and/or rna molecules. Detection of specific dna fragments or rna transcripts could be much more problematic when they are present in a mix with hundreds of other dna or rna molecules. Blot hybridization is a method used in molecular biology for detection of specific. Methods of blotting involve the transfer of electrophoretically separated molecules from the gel onto a nitrocellulose or nylon membrane. Southern blotting (named after edwin southern) is the term for dna transfer. After blotting, particular dna or rna molecules on the membrane can be detected via hybridization with a dna probe that specifically binds to the target molecule. Probes are used to identify specific targets from a large pool of heterogeneous molecules separated in gel electrophoresis. The pairing of complementary sequences between the probe and target. Northern blot hybridization nucleic acid is called hybridization. Northern blotting and hybridization with the probe can locate specific transcripts in a large pool of rna.
