BIOL 039 Lecture Notes - Lecture 28: Cloning, Molecular Genetics, Moe Williams
Document Summary
Molecular genetics is the field of biology that studies the structure and function of genes at a molecular level and thus employs methods of both molecular biology and genetics. The development of recombinant dna technologies and the polymerase chain reaction (pcr) Make it possible to locate, isolate, amplify, alter, and study specific dna segments. To study a gene at the molecular level you need. First, identify a dna fragment that carries that gene; and second, isolate this fragment in sufficient amounts to be useful for further analysis. The procedure of isolation and amplification of a given gene is called gene cloning. Gene cloning relies on recombinant dna molecules those that are made by joining two or more different dna molecules. Cloning of a gene or any dna fragment allows to amplify this gene or dna fragment. There are two ways of dna amplification in vivo amplification in vitro amplification.
