MICRB 107 Lecture Notes - Lecture 2: Flagellum, Iodine, Lipopolysaccharide

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orchidleopard260

20 Sep 2017

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The refractive index is a measure of the light-bending ability of a medium. The light may bend in air so much that it misses the small high-magnification lens. Resolution (resolving power) is the ability of the lenses to distinguish two points. A microscope with a resolving power of 0. 2 um can distinguish between 2 points >_ 0. 2. Immersion oil is used to keep light from bending um. Shorter wavelengths of light provide greater resolution. You can use oil to improve resolution also a blue filter can help as well. Illuminator condenser lens specimen objective lenses prism ocular lens . Dark objects are visible against a bright background. Light reflected off the specimen does not enter the objective lens. Objective low, high dry, oil immersion total magnification: ocular magnification * objective magnification, stains improve contrast * Light objects are visible against a dark background. Light reflected off the specimen enters the objective lens.

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QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

limecheetah620

QUESTION 1

The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---

	a.	10x
	b.	20x
	c.	100x
	d.	1000x

QUESTION 2

The part of the microscope that focuses the light on an object is the ___________.

	a.	Iris Diaphragm
	b.	Coarse Focus
	c.	Condenser
	d.	Body Tube

QUESTION 3

The polarizing filter in a PLM that is located above the objective is called the _________________.

	a.	Polarizer
	b.	Analyzer
	c.	Abbe condenser
	d.	Iris

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

1 points

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

maroonmuskrat824

Circle the correct answer from choices shown in bold or fill inthe blank:

1-When necessary, adjust the intensity of the slideâsillumination using the light source / condenser.The contrast of the image can be adjusted using theiris diaphragm / condenser.Contrast is (briefly explain in one sentence):__________________________________________________________________.

Loss of contrast usually results from too little / toomuch illumination.

2. Never use the 4X / 10X / 40X / 100Xobjective without immersion oil. In this course, will you be usingthis objective? Yes / No.

3- Before removing a slide from the stage, lower /raise the stage and return to the 4X / 10X / 40X /100X objective. When completely finished using themicroscope, leave the light source on / turn off the lightsource.

4. Give the numerical aperture values for the followingobjectives (get these values from the microscope in front of you):4X _______, 10 X _______ and 40 X _______. Numerical aperture meanswhat? __________________________________________.

5-The ability to perceive or distinguish two adjacent points asseparate entities on an image is called ____________. It used as anindicator of lens / specimen quality. An increasein magnification can lead to a reduction in ____________ and____________.

5. What does the term parfocal mean in microscopy? Give a briefdescription.______________________________________________________________________

skyfrog42

MICRB 107 Lecture Notes - Lecture 2: Flagellum, Iodine, Lipopolysaccharide

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