01:119:115 Lecture Notes - Lecture 17: Polymerase Chain Reaction, Polyacrylamide Gel Electrophoresis, Molecular Sieve
18 Dec 2016
School
Department
Course
Professor
Document Summary
Lecture 17 - dna technology: dna sequencing, ingredients for dna sequencing. What you need for dideoxy chain termination method: 1 fragment of dna to be sequenced - many copies. Primers - short segments with known 3" ends. Polyacrylamide gel - acts as a molecular sieve. Take all fragments and run through a. Results presented as a spectrogram: uses for sequencing. Discover the sequence of a dna fragment. You will do this in bio 117. Making multiple copies of a gene or a dna segment. Problem: there is often not enough dna to run many tests. Gel electrophoresis: pcr - polymerase chain reaction (patterned after dna replication) Method to produce billions of copies in a short period of time. Helps study dna and read small samples. 3-step process that is repeated 30-40 times: Hydrogen bonds are weak and break at high temps. Hydrogen bonds separate, phosphodiester bonds do not. Heat > 90 separates dna into two strands.
