01:119:131 Lecture Notes - Lecture 3: Crystal Violet, Mycoplasma Pneumonia, Mycolic Acid
9 Dec 2016
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Document Summary
Helps maintain integrity of cell when cell is under diff osmotic pressure. Hanging off of sugar is 4 amino acids. *difference between the two is thickness of gram layer; how many layers found outside. Typical gram-positive bacteria peptidoglycan layer is right outside cytoplasmic membrane. Bacterial cell needs to get food between membrane. Peptidoglycan doesn"t let alc solution release from cytoplasm. Space that we don"t see in gram (+) organism. Extra membrane outside slim peptidoglycan layer: outer membrane. Lipopolysaccharide molecule (lps) *only found in gram (-) Problem when treating people with gram (-) infection. Cell is dying/falling apart, lipid a is released. Since peptidoglycan layer is so thin, crystal violet dye comes out. Staining has to do with thickness of peptidoglycan layer. Difficult to get anti bacterial to this waxy, thick layer (waxy looking layer) Red dye (carbofusion) can enter the cell; treat it with acid wash. Acid-fast stain is required when trying to make diagnosis.
