01:447:380 Lecture Notes - Lecture 13: Seymour Benzer, Coinfection, Wild Type
Document Summary
Infect e. coli k12 with 2 different rll mutant phage. Bacterial cell is now diploid for all phage genes. Group a strain and group b will complement each other. Rll mutant strains in grp a have mutation in gene a. Rll mutant strains in grp b have mutation in gene b: recombination experiment. Coinfect e. coli with 2 rll mutants from either grp a or b. Hypothesis: intragenic (within 1 gene) recombination can happen. Give distance between 2 mutation sites that are within the same gene recombinants. # of plaques on b = total. # of plaques on k = of total. If no recombination, then we see no prototrophs and mutations must be in the same spot within the same gene. Work should that the gene is divisible. Gives order of intermediates in biochemical pathway; determines which step of the pathway is blocked in each strain. Supplement min media with different intermediates in biochemical pathway (a,b,c,d,e)
