01:447:380 Lecture Notes - Lecture 15: Kozak Consensus Sequence, Nucleic Acid Sequence, Ribosome
Document Summary
*** ribosome reads mrna and join amino acids together. *** trnas bring amino acids to ribosomes trna. Process of attaching the correct amino acid to its trna. There are 20 (1 for each amino acid) Recognize base modifications unique to different trnas. There are >20 trnas (1 synthetase may need to recognize multiple trnas) Mutation in anti-codon loop will synthetase does not read sequences of codons. Not affect charging, because the comprised of protein and rna: small subunit of ribosome binds mrna; has small and large subunits, small subunits: prokaryotes - 30s & 50s, eukaryotes - 40s & b. Lines up ribosome for initiation at aug: eukaryotes: no shine-dalgrano; ribosome binds 5"cap & scans mrna to find aug; kozak consensus sequence around aug (used to help subunits find aug) fmet-trna met a, met-trna met . Eukaryotes: 1st aa-trna binds mrna (initiation factors help); carries methionine. Initiation factors are released & large ribosomal subunit binds.