01:447:484 Lecture Notes - Lecture 2: Microtubule, Nuclear Transport, Nocodazole
18 Jun 2016
School
Department
Course
Professor
Document Summary
Crtc1 is going from synapse to the nucleus. its a transcription factor. when dephosph, it goes to nucleus. 5 different experimental conditions: how is the crtc1 moving from synapse to the nucleus context fear conditioning, no cue. This is dependent on 2 structures: amygdala and hippocampus. 1 shock: mouse in box for 2 mins, and then shock is given. 3 shocks: 3 shocks are given intermediate shocks: mouse gets into box and get shock right away. home cage: never been in box context: sit in box, but nothing happens to it difference between home cage and context only: Stronger memories is associated with increased crtc1 nuclear localization in ca1 and. Ca3, not dentate gyrus. most increased amount of crtc1 is after 1 hour of training. Next experiment: only 3 controls instead of 5: Whole extract shows no change after training learning-induced nuclear accumulation crtc1 is independent of rna transcription. Learning induced decrease of crtc1 in the psd.
