MLS 341 Lecture Notes - Lecture 3: Personal Protective Equipment, Marburg Virus, Mycobacterium Tuberculosis
Document Summary
Upon completion of this lesson, the student will be able to: Define biosafety levels 1 through 4; including the precautions required for each; identify a representative organism for each level. Level 1: no known potential for infection. standard laboratory techniques. Level 2: common agents of disease primary containment equipment is used. Sterilization: process that kills all forms of life incineration is most common method, temps around 870 c, safest to ensure no infective material. Moist heat: steam under pressure, auto clave (steam enters through top and pushes air out bottom: gravity displacement) Dry heat: longer exposure and higher temperatures, sterilize glassware, oil and petroleum or powders with a hepa filter. Filtration: method choice for antibiotic solutions, hepa filters designed to remove organisms larger than 3 nm from isolation. Ionization radiation: ex: microwave, short wavelengths and gamma rays used to sterilize disposables. Disinfection: process during which most pathogenic organisms are destroyed. Boiling at 100 c for 15 minutes, kills vegetative bacteria.
