MLS 341 Lecture Notes - Lecture 6: Screw Cap, Gram Staining, Methylene Blue
Document Summary
Objectives: upon completion of this lesson, the student will be able to: State the appropriate media for primary plating, specimen preservation, storage and transport of frequently requested cultures. (i. e. ; throat, wound, urine, wound, stool, sputum, blood etc. ) Throat: swab moistened with stuarts or amies medium, <2hr, 24 hour storage , plate on bap or. Wound: aerobic swab moistened with stuarts or amie"s medium, patient preservation: wipe area with sterile alcohol, <2hr, 24hr storage, plate: bap, mac, cna, anaerobic bba, direct examination: gram. Blood/bone marrow: blood culture and vacutainer with sps, disinfect site with alcohol, draw blood and do w/in 2hrs must be incubated 37c, plate: bap, bba anaerobic, direct gram stain from positive blood cultures. Bone: sterile and screw cap, disinfect skin before procedure, take sample from affected area, immediately report, plate asap, bap, mac, thio, gram stain. Csf fluid: sterile screw cap, disinfect skin before aspirating specimen, rapid testing, <15minutes or incubate 37c, bap, thio, gram stain.
