BIOL 100 Lecture Notes - Lecture 14: Ecori, Plasmid, Sanger Sequencing

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23 Oct 2017
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Biology 100 - lecture 14 - dna technology. In the 1970s biologists discovered bacterial enzymes that allowed them to cut and paste. Molecular biology experiments were put on a moratorium to assess the ethics of dna recombination. Ultimately, this discovery led to the process of dna transformation, the production of transgenic organisms and ultimately dna-based technologies. In !970, paul berg produced a recombinant dna for the first time as he stitched together. Dna from two viruses with 3 genes from e. coli. Janet mertz inverted berg"s experiment-clones viral dna into e. coli genomes. In 1972, mertz used an enzyme from bacteria, ecor1 to clone dna that was isolated by. They protect bacteria from viruses and bacteriophages. First isolate the genomic dna of interest. Cut the plasmid and dna from the human cells. Paste the human dna into plasmid which is called recombinant dna. Transform the dna into bacteria by heat shock.

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