FS 160 Lecture Notes - Lecture 13: Base Pair, Zygosity, Lysis

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8 May 2018

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FS 160 – Lecture 12 – Databases

I. DNA Analysis Workflow

a. Sample collection and storage

b. Extraction and quantitation

c. Multiplex PCR amplification

d. Capillary electrophoresis and data interpretation

II. Limitations of Current Technology

a. Low input samples

i. May yield insufficient material for multiple tests

ii. Single test yields insufficient information to resolve case

b. Mixed samples

i. Current length based methods limit resolution of complex mixtures

ii. Suspects evade conviction, fail to be exonerated, or offenders remain

undetected

c. Compromised samples

i. Current systems cannot combine large numbers of short markers

ii. Partial profiles are common, limiting the power of the result

III. Forensic Questions

a. Does the sample contain biological material?

b. What is the cellular origin of the biological material?

c. When was the material deposited?

d. What was the age of the donor?

e. Who is the donor of the biological material?

f. Are the crime and the biological material related?

IV. Current Forensic Workflow

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