MLS 301 Lecture Notes - Lecture 1: Hemoglobin, Sodium Dithionite, Monocyte
Document Summary
Objectives: upon completion of this lesson, the student will be able to: * normal vs abnormal development, function, reaction to disease, physiology. Describe the process and method for making a peripheral blood smear (wedge method) page 237. Drop of blood, take one slide at 30-45 degree angle and smear, get feathered edge. Describe the characteristics of a properly prepared peripheral blood smear. Feathered edge, not too thin or too thick, 2/3 to 3/4 length slide, finger shaped, film is visible, smooth, no holes, tears or streaks. Explain the staining process using a wright stain and name all of the components. Romanowsky type stain, first step is methanol fixation step. Next slide is stained with methylene blue and eosin stain. Describe the characteristics of a properly prepared peripheral blood stain. Not too blue or not too pink, no presence of precipitants. Given an improperly stained peripheral smear, discuss the causes and appropriate steps required to correct the problem.
