BIO 314 Lecture Notes - Lecture 3: Fusion Protein, Myc, Chronic Myelogenous Leukemia

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Chapter 4: cellular oncogenes: understand how transfection, hybridization and dna sequencing led to the identification of non viral oncogenes. Gene transfer of foreign dna that can be expressed in a eukaryotic cell. This is used to create stable genetically modified cells. Dna of chemically transformed cells was injected into a mouse host to induce tumors. Hybridization- finding dna sequence of interest by complimentary base pairing. Denaturing and reannealing h bonds allows dna from two sources to be combined. Probes need to be able to base pair and have a means of detection. Dna sequencing- looking for the specific mutation that caused oncogenic activation. G12v mutation was responsible for ras oncogene activation. 25% of cancers have this ras mutation: understand what is meant by structural and regulatory alterations, and give examples of each. Normal protein greatly overproduced: understand how techniques such as fish, ihc, and microarrays are used to characterize non viral oncogene activation in human cancers.