NEU 211 Lecture Notes - Lecture 6: Antibody, Phenoxybenzamine, Atropine

• School: Syracuse University
• Department: Neuroscience
• Course: NEU 211
• Professor: Jacob Shreckengost

Document Summary

List of neurotransmitters thought to function in the cns is ~50 at last count. Many have different receptors that allow different effects in different locations, using the same neurotransmitter. Can use immunologic approach or in situ hybridization approach to identify proteins involved: if removal method involves re-uptake and protein that re-uptakes is from somewhere else. Use also immunologic approach or in situ hybridization approach to identify proteins involved. **methods that allow us to see whether a neuron contains/synthesizes a transmitter candidate. A molecule must have same localization, synthesis, and release to be the same as a transmitter. In situ hybridization [molecular biological]: method used to confirm that a cell synthesizes a particular protein or peptide. A strand complementary to known strand of mrna is made = probe. Probe bonds to the mrna molecule to see if mrna for particular peptide is localized in a neuron.