BIOL 111 Lecture Notes - Lecture 2: Plasmolysis, Euglena, Water Potential

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15 May 2016
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Ph meter=quantitative bc most accurate qualitative vs. quantitative termites types of transport. What lugol"s tests for: starch and understand materials, control, experiment, and observations look @ slides on cells** all experiments done in class. Plasmolysis: water potential movement, hyper, hypo, and isotonic sol"ns. Spectrophotometer: basic parts and function; how to use; how to blank-purpose, concentration inc=rate of rxn inc=amt of product formed inc=absorbance inc, catecholase=enzyme, substrate=catechol; where does it bind? how does it catalyze rxn, variables inc until maximum velocity reached. Enzyme becomes limited after certain point-active site becomes only substrate; no more for enzyme to bind to. Ph and temp: have optimal range under which activity of enzyme=max above or below=denatured. Humans=7. 48 ph-small diff. big changes in diseases, etc. cells: diff. in microscope and models of prokaryote vs. eukaryote. Prokaryotes have dna in nucleoid region, not bound by membrane: plant vs. animal. Euglena cyanobacteria: endosymbiosis-mito. and chloroplasts, gram +/- bacteria: due to peptidoglycan.

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