BIOL 351 Lecture Notes - Lecture 3: Lysine, Yeast Extract, Neutral Red
21 Mar 2018
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Results (indicate appearance of positive/negative reaction) do not write name of organism. Red slant/no change in butt (k/nc) a/a, k/k, h2s, nc/nc, k/a. Red complex due to the indole"s reaction with the aldehyde group in the kovac reagent; cherry red layer forms on top of medium. Purple (first yellow due to drop of ph because of glucose fermentation, but putrescine raises ph changing color to purple) Gram positive or gram-negative bacteria based the composition of their cell walls. Crystal violet, iodine (enhances crystal violet stain), ethanol/acetone (decolorization), safranin (counterstain) Semi-solid consistency of medium cloudy (organism is motile) For organisms that can ferment glucose and covert products to. Reagent a (alpha naphthol & absolute alcohol) & reagent b (potassium hydroxide & distilled water) Sodium malonate, yeast extract, small amount of glucose, bromothymol blue dye. Organism reduces nitrate to something other than nitrite. Reagent a (sulfanilic acid & 5n acetic acid) & reagent b (n,n-
