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Class Notes for Genetics at Texas A&M University

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GENE 320 Lecture Notes - Lecture 21: Genbank, Sanger Sequencing, Protein Structure
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- Used for analyzing proteins FISH involves hybridizing probe directly to chromosome or RNA without blotting Sanger method: Dideoxynucleotide - Hydrogen at 3 instead of a...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 22: Pyrosequencing, Peptide, Sanger Sequencing
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Lecture 22: Thursday, April 13, 2017 at 1109 AM Protein coding regions: Annotation of sequence reveals several identiable features indicating that the sequence contains a protein coding gene ...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 19: Tumor Suppressor Gene, Red Meat, Ultraviolet
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mutations in proto-oncogenes and tumor supressor genes are necessary for full malignancy Carcinogens: Any substance or event that damages DNA and causes mutations to occur in proto-oncogenes or tumor s...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 17: Myotonic Dystrophy, Five-Prime Cap, Galactose
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promoter bound factors - Coactivators form complex enhanceosome - Enhanceosome** - intereacts with transcription complex ...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 17: Chorionic Villus Sampling, Statistical Hypothesis Testing, Trisomy
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Represent quantitative differences in number of large DNA sequences Found in coding and noncoding regions of genome Play crucial role in expression of traits Duplications are the primary so...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 24: Comparative Genomic Hybridization, Whole Genome Sequencing, Copy-Number Variation
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ASO used for PGD GeneChip methods** Comparative genomic hybridization (CGH) microarray techinque used to identify copy number variation throughout the genome Whole genome Transcriptome a...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 20: Molecular Cloning, Complementary Dna, Bacteriophage
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Carries a higher acount of kb (45) than most smaller replication vectors - Larger replications vectors - BAC & YAC Expression vectors: Designed to ensure mRNA expression of cloned gen...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 18: Hereditary Nonpolyposis Colorectal Cancer, Chronic Myelogenous Leukemia, Xeroderma Pigmentosum
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In colorectal cancers, adenoma or polyp formation requred inactivation mutation in ADP gene Carcinogens = cancer causing agents ex: radiation requires a multistep process to s...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 23: Homology Directed Repair, Crispr, Nuclease
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Cre-Lox system Used for making conditional KOs - Allows scientist to control when target gene is disrupted - Inserted into the germ line Knock-in animals/Transgenic animals...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 13: Aminoacyl Trna Synthetase, Rna Editing, Aminoacylation
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Splicesome Pre mRNA introns spliced out by spliceosome reaction involves formation of lariat structure RNA editing substitution editing - Indentities of individua...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 14: Joshua Lederberg, Petri Dish, Bacteriophage
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Radical group bound to central carbon atom - determines type of amino acid - hydrophobic - hydrophilic - positive or ne...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 15: Dna Mismatch Repair, Ames Test, Proofreading
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Can substitute for purines or pyrimidines during nucleic acid biosynthesis Increase tautomeric shifts Increase sensitivity to UV light - mutagenic - Ex: 5-br...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 12: Open Reading Frame, Phosphodiester Bond, Pyrimidine
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Pseudogenes - large number of single copy noncoding regions - genes whose sequences have degenerated sufciently during evolution to render them nonfunctional DNA sequences representing evolutionary vestiges ...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 10: Chromosome, Microrna, Small Nuclear Rna
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find more resources at oneclass.com Lecture 10: Genetic Material Tuesday,February21,2017at10▯32AM Genetic material criteria: • Replicate • store information • express information ...

Genetics
GENE 320
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GENE 320 Lecture Notes - Lecture 9: Symbiogenesis, Gamete, Zygosity
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find more resources at oneclass.com Lecture 9: Maternal Inheritance Tuesday,February14,2017at11▯09AM Dosage Compensation questions on test* Extranuclear inheritance • inheritance of genetic informati...

Genetics
GENE 320
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