BIOL 1001 Lecture 19: DNA Technology & Genetic Engineering
Document Summary
Biol 1001 lecture 19 dna technology & genetic engineering. Making recombinant dna: restriction enzymes discovered in late 1960"s, cut double-stranded dna into fragments, cleave bonds in dna backbone (sugar phosphate, these enzymes do not end in the suffix -ase, fragments base-pair at sticky ends. Enzyme for pcr: the enzyme used for pcr is called taq dna polymerase. Traditional dna sequencing: once you know the dna sequence, can develop a genetic test huntington"s disease, Pku, etc: nucleotides are assembled to create complementary strands, when a modified nucleotide is included, dna synthesis stops. If dna sequence of a gene is known may predict efficacy of a drug: pharmacogenomics, herceptin is an antibody, her2-rec positive and her2-rec negative breast cancer. Human genome project & dna sequencing: also known as hugo, goal map the entire human genome all 24 chromosomes 22 autosomes, x and y chromosomes, used technique known as dideoxy sequencing. Initially thought by many to be a waste of resources.