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Lecture 9

BIOL 3096 Lecture 9: 9


Department
Biology
Course Code
BIOL 3096
Professor
Joel Sheffield
Lecture
9

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ProteinfoldingVideos
Thebestobtainedstructurethroughacomputerprogramischeckedagainstthecrystallographicdata
Aromaĕcresiduescreateahydrophobiccore
Insulin:excepĕontotheproteinfolding
Can'tdenatureandreform
Ithas2chains,αandβconnectedthroughdisulfidebonds
Insulinissynthesizedasasinglelongchainedpolypepĕdecalledproinsulin
Thisfoldswithinitselfandisthencleavedbyanenzyme
Likeascaffoldthatistakenoutbytheenzyme
HeatShockProteins
Createdwhenanimalsareheatedtoalevelwheretheyarestressedbutnotdead
Createdatlowdosesinunstressedcells
Theyarenecessarytoallowotherproteinstofoldproperlyduringstressbyshieldingthem
ChaperoneProteins(Chaperonins)helpguideformaĕon
Normalaminoacidchainsarecapturedbythissetofproteinsthatholdtheminplaceunĕltheycan
foldedintotheirproperconfiguraĕons
Ifthisisnotpossiblethentheyarethrownout
Usedtoselect/discard
Reasonwhytherearenotmanynon‐funonalproteinsinanyanimal
I.e..Proteindisulfideisomerase
SeparaĕonTechnology
ApplyaForcetoamixtureofmaterials,providearesistancetotheforcethataffectsdifferent
componentsdifferentlyandhavesomewayofcollengtheseparatedmaterial.
Properĕesthatcanbeused
Size
Density
Charge
Mass
Forcesthatcanbeused
Fluidflow
Gravity‐density‐exaggeratedwithacentrifuge
Electriceld‐charge
SizeExclusionchromatography
Separatebasedonsize
Hydrodynamicradius
Amountofspacethattheytakeupinwater
Forceusedistheflowoffluid
Resistance:Sephadex‐beadscreatedbyPharmacia
Gelhasamatrix
Thelargemoleculescan'tgetin
Smallermoleculescangointhroughpores
Bigthingsthuscanmovethroughalotfaster
Lecture9
Monday,September14,2015 10:45PM
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