BIO-0013 Lecture : 11-29-12.docx
Document Summary
11 29 12: run northern blot with complementary probe for gene x, run another northern blot when enhancer is removed, see difference in the amount of rna made, decreased rna on northern blot when enhancer is removed, enhancer experiment, studied immunoglobulin heave chain/antibody gene found in b cells, b cells make antibodies, wanted to see if they could take enhancer elements out and still get rna for antibodies, human gene for antibodies. Cell 4 not detectable: to regulate transcription of a gene, specific regulatory proteins need to bind to the correct dna sequences (enhancers, silencers, ppe, how can you test if enhancers are binding sites for regulatory proteins that activate transcription? o, can a b cell enhancer work on a rbc gene, b cell with just beta globin gene, b cell with beta globin gene and b cell enhancer, on northern blot.
