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BIO-0013 (103)
Mc Vey (25)
Lecture

11-13-12.docx

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Department
Biology
Course
BIO-0013
Professor
Mc Vey
Semester
Fall

Description
11­13­12 • Gene expression: the process of translating the information in DNA into  functioning molecules within the cell • How are DNA sequences related to phenotypes? o Genotype  ▯phenotype o All information is in the genotype, but all the information is not being  used at once in the phenotype o Environmental variation caused by diet, lifestyle, temperature • What do genes do? o 1 gene, 1 enzyme hypothesis o Concept: figure out what something does by “breaking it”  Use neuraspora crassa  Stage in lifecycle where it is haploid • Don’t have to consider dominant/recessive  If you knockout a gene, what is the phenotype? o Wild type mold can be given precursor and make its own arginine to grow o Mutants cannot grow when given only precursor  Hit with x rays to create mutations  Put spores into complete nutrient medium where they can grow  Transferred from complete to minimal medium • Whichever tube doesn’t grow is an auxotrophic mutant  Grow in minimal mediums with single amino acid • Whichever it grows in is the one it is deficient in making • To decide where the problem is in the metabolic pathway o Add precursors one at a time and see when it starts being able to form the  product o When it grows, that means the enzyme that makes whichever precursor  you added is the defective one • Central dogma of mole
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