BIO-0013 Lecture Notes - Telomerase, Topoisomerase, Primase
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29 Mar 2014
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Needed for dna: helicase, primase, dna polymerase iii dntps topoisomerase, ssb proteins, dna ligase, telomerase (eukaryotes, clamp, dna polymerase i, dna template, highlighted items are the ones absolutely necessary for dna synthesis. Dna can be made single stranded by dna helicases, or by heating to temperatures that disrupt the hydrogen bonds: the logic behind pcr, denaturation of dna duplex (94degreesc, annealing of primers (55 60degreesc, dna synthesis by polymerase (72degreesc) This was solved with the discovery of a bacterium named thermos aquaticus, which makes a polymerase that is stable at high temperatures: taq polymerase, desired dna product (without excess of the dna that you don"t care about) does not appear until cycle 3, to visualize the products of a pcr reaction, you use gel electrophoresis with agarose gel, you use pcr for basically any situation where you have very little dna and need to make more copies for further analysis.
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