BIO-0046 Lecture Notes - Lecture 3: Tumor Suppressor Gene, Sh3 Domain, Reverse Transcriptase
Document Summary
Lecture 3: receptor tyrosine kinases o o o o o o o o o o o o o. You identify a wildtype (wt) tumor suppressor and find it regulates src. The sequence of the tumor suppressor reveals that it has a phosphatase domain. Tumor suppressor = decreased activity of the tumor suppressor gene product leads to cancer (tumor suppressors wants to inhibit growth) (tumor suppressor wants to inhibit src) We want tumor suppressor gene to be on, so kinase. When kinase domain tyrosine is phosphorylated, results in increased tumor suppressor gene product. Src wt, src wt -----gof----> src wt, src * Src wt, src wt ----lof---> src wt, src * -----lof---> src *, src* With one copy gone, there"s still enough regulatory activity left with half of the wt src. Both copies need to be gone to affect the cell. If there"s a gof mutation on a tumor suppressor, it would make a better tumor suppressor!
