BIO-0013 Lecture Notes - Prnp, Proteasome, Chaperonin
30 Jan 2014
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Document Summary
Western blots: like northern blots, but that looks at proteins, westerns look at proteins, technique for detecting specific antigens separated by electrophoresis by use of labeled antibodies, take a population of cells and isolate the protein in the cell, run the proteins on a gel thinner than agarose gel, sorts out proteins in matrix by size, transfer proteins to nitrocellulose membrane, need energy for this transfer, not chemical reaction like northern, shoot some electricity into the gel being covered by cathods, end result is proteins on the membrane, way to visualize proteins, identify your protein of interest, instead of using probe of nucleic acids, use an antibody from cells. Application of rnainterference: microrna interfere with the mrna and fuck its shit up, way of getting rid of rna, scientists can make synthetic silencing rna, seeing if you can get rid of specific rna messages to stop production of a protein, macular degeneration debilitating eye disease.
