BSC 300 Lecture Notes - Lecture 17: Microtubule Nucleation, Fluorescence Microscope, Atomic-Force Microscopy
21 Oct 2016
School
Department
Course
Professor
Document Summary
A network of filamentous proteins structures: microtubules, microfilaments and intermediate filaments. Each is a polymer of protein subunits held together by weak, non-covalent interactions. Each is highly dynamic: capable of rapid assembly/disassembly. Scaffold providing structural support and maintaining cell shape. Directs cellular locomotion and the movement of materials within the cell. Dynamic change in cell shape and principle force of cell motility. Internal framework to organize and position organelles within the cell: anchoring sites to asymmetrically distribute mrnas, promote signal transduction in cell signaling, esse(cid:374)tial (cid:272)o(cid:373)po(cid:374)e(cid:374)t of the (cid:272)ell"s di(cid:448)isio(cid:374) (cid:373)a(cid:272)hinery. Dynamic processes can be visualized in vivo. Right, rapid changes in length of microtubules revealed in time lapse movie through rhodamine conjugated tubulin monomers. Rhodamine: small, fluorescent dye that can be covalently linked to proteins. Can detect activity of an individual protein molecule in real time. Video microscopy can be used to study the dynamics and speed of motor proteins.
