BSC 300 Lecture Notes - Lecture 7: Complex System, 3D Projection, Antibody
7 Sep 2016
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Document Summary
Define magnification and resolution for light microscopy. Describe methods for improving resolution in light microscopy. Outline different light and electron microscopy techniques emphasizing the information they reveal and their advantages and disadvantages. Robert hooke first observed and named cells in 1655 using an elegant compound microscope with a magnification power of only about 50x. A simpler microscope using a single, small spherical lens. Increased magnification to almost 250x and eliminated aberrations and distortions. He described a miniature world of single cells organisms he called animalcules (protists) Lenses of a compound scope: condenser lens: focuses light, objective lens: magnifies and establishes resolution, ocular lens: empty magnification. Total magnification is the product of the magnification of the objective and ocular. It is dependent on curvature and composition of the lenses. Objective lens composition, size and shape determine degrees of magnification and resolution. Resolution: the shortest distance between two points on a specimen that can be distinguished as separate entities.
