BSCI 330 Lecture Notes - Lecture 2: Sodium Chloride, Microscope Slide, Dna Gyrase
5 Oct 2017
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Document Summary
The nucleus was discovered in 1831 by robert browne through microscopic observations. 2 layers, a perinuclear space exists between the two layers continuous along the pore complexes. Is also continuous with the rough er. Provides structure to the envelope and organizes chromosomes. Prevents solubilization when the nucleus is in a detergent. Rer contains membrane bound ribosomes that produce secretory proteins. To procure the nucleus in the first place, a technique called differential centrifugation is used to separate out the different components in the cell. Cells suspended in solution are mechanically disrupted and fractions are collected through sequential steps of centrifugation. Large organelles move faster than small organelles and thus are collected first. The nuclear lamina is comprised of two proteins: lamin a and lamin b (two genes each) During mitosis, phosphates bind to lamin and the nuclear membrane is fragmented. Phosphates are then removed by enzymes to reorganize membrane. Mutations in lamin a/b genes leads to premature aging syndrome (hutchinson-gilford.
