not too details, just main points please...
1. What you should know about microtubles
- MT structure, what they are made of
- MT function
- Motor proteins(kinesins&dyneins)
- How kinesins move, coupling to ATP hydrolysis
- Microtubule Organizing Center (MTOC), how MTs are nucleated
- MT dynamics, how it's affected by drugs and MAPs
- dynamic instability and how its linked to GTP/GDP cycle
- cilia/flagella, axoneme structure
- how cilia/flagella move and the role of dynein
-intra-flagellar transport
2. What you should know about actin filaments
- F-actin structure
- F-actin Function
- Motor proteins (myosins)
- Actin dynamics, how it's affected by actin-binding proteins
- the concept of critical concentration
- actin/myosin structure and function in the contezt of muscle
- how muscle contract
- actinomyosin cycle (myosin movement), coupling to ATP hydrolysis
- How cells move and change shape, using actin
3. What you should know about IF
- IF strcuture, what they are made of
- IF function, roles inside and outside cells
- Regulation of nuclear IF in the cell cycle
- be able to compare major features of IF, Actin, and MTs
Bacterial cytoskeleton, that bacteria have proteins that
- polymerize
-affect cell shape, cytokinesis, ect.
- play analogous roles to MT, F-actin
4. what you should know about the cell cycle
- parts of the cell cycle, stages of mitosis
- MPF; how it was identified, what it is made of (cyclin and cdk)
- Universality of cyclin/Cdks (in yeast, frogs, etc.)
-how Cdk activity is regulated (many mechanisms)
- how Cdks function (by phosphorylation)
- how multiple cdks divide their function
- how the spindle is formed and mistakes corrected
- the role of motors and MY dynamics in above
- the tolr of proteolysis in mitotic transitions
- what a checkpoint is
- how checkpoints provide an additional layer of cell cycle regulation in case of damage