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Lecture 1

BSCI 223 Lecture Notes - Lecture 1: Citric Acid, Organism, Motility


Department
Biological Sciences Program
Course Code
BSCI 223
Professor
mciver
Lecture
1

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Group 5
Aji, Sammy, Jessi, Jenna
PHENOTYPIC DATA
TABLE 1: Results from phenotypic analysis of Organism A.
Test Data from
study of
your
isolate
Interpretatio
n of data
Comment
1.Colony
characteristics on
TSA
positive Bacteria
prefers solid
media
Lots of growth
2.Simple stain –
shape/arrangement
N/A N/A Washed of cells
3.Gram Stain Stained
purple
Gram
positive
Cells remained stained purple from
crystal violet
4.Endospore Stain positive Endospores
present
Green sections inside cells
5.Motility Growth of
bacteria
straight
down
Not motile Bacteria grew along path of needle
from stab and didn’t move around
tube
6.Hemolysis on
Blood agar
positive beta A bit cloudy, but somewhat
transparent almost color of the
medium
7.Growth on
MacConkey
positive Gram
negative
There was pink growth, but white
growth in the center so maybe there
was some contamination
8.Lactose
Fermention/
MacConkey
positive Lactose
fermenting
bacteria
Pink growth
9.Growth
characteristics:
Temperature
4° = -
25° = +
30° = +
55° = -
mesophile a lot of growth in the plates under
25 and 30 degrees
10.Growth
characteristics:
Oxygen
requirement
positive Aerobic
microbe
There was growth both in the NA
Deep and the aerobic environment
11.Growth
characteristics: Salt
tolerance
7.5% = +
10% = +
15% = -
20% = -
Salt tolerant The plates that contained growth
had very weak growth
12.Growth
characteristics:
Minimal media with
glucose
negative Doesn’t like
glucose as a
carbon
source
There was no growth
13.Growth
characteristics:
positive Prefers
citrate as
About 90% of tube was blue,
indicating that my organism really

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Simmons citrate carbon
source
like citrate as carbon source
14.Fermentation;
Glucose
negative Acid not
present,
doesn’t
ferment
glucose
Cloudy at top of tube indicating
growth, no color change
15.Fermentation:
Lactose
positive Acid present,
ferments
lactose
Growth in tube, liquid changed to a
darker cloudy orange color
17.Oxidase test positive Oxidase
positive,
aerobic, can
use O2 as e-
acceptor in
respiration
dark blue color after ~10sec
18.Catalase test positive Catalase
positive,
grows in an
oxygen rich
environment
Bubbled a lot and continuously
TABLE 1: Results from phenotypic analysis of Organism E.
Test Data from
study of your
isolate
Interpretatio
n of data
Comment
1.Colony
characteristics on
TSA **
Yellowish
circular
colonies
2.Simple stain –
shape/arrangement
Inconclusive –
cells washed
off
n/a Only stain could be observed, so I
believe I did the simple stain
incorrectly.
3.Gram Stain Pink cells with
thin cell wall
Gram-
negative
bacteria
4.Endospore Stain No sporulation
observed
Endospore
negative
Not fully confident in the accuracy
of this test as I do not know if I did
the endospore stain correctly.
5.Motility Isolate spread
throughout tube
Positive for
motility
Potential indicator of flagella
6.Hemolysis on
Blood agar **
Clearish/pinkish
growth
Gamma
hemolysis –
lack of
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