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Lecture 1

BSCI 223 Lecture Notes - Lecture 1: Transmission Electron Microscopy, Microscopy, Nucleoid

Biological Sciences Program
Course Code
BSCI 223

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4. Using light microscopy, you examine the soil samples
and the 'goo' from the degraded polyurethane. Will this
approach allow you to observe all microorganisms
present in the samples?
Why or why not?
What are the limitations of this approach?
Light microscopy has a less efficient zoom capacity compared to higher grade
microscopy such as a scanning or transmission electron (which zooms more). Anything
smaller than a certain range of nanometers will require more magnification than what
light microscopy is capable of because its size is unable to reflect the light. One can only
make limited observations based on the surface rather than the internal structure of the
organisms, as well. In the case that the microorganisms may be alive, transmission
electron microscopy can be utilized in place of scanning, which requires dead specimens.
These two options of electron microscopy provides a wider range of success in
observation than light microscopy. [BSCI223 lecture]
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