BSCI 223 Lecture Notes - Lecture 1: Transmission Electron Microscopy, Microscopy, Nucleoid

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Using light microscopy, you examine the soil samples and the "goo" from the degraded polyurethane. Light microscopy has a less efficient zoom capacity compared to higher grade microscopy such as a scanning or transmission electron (which zooms more). Anything smaller than a certain range of nanometers will require more magnification than what light microscopy is capable of because its size is unable to reflect the light. One can only make limited observations based on the surface rather than the internal structure of the organisms, as well. In the case that the microorganisms may be alive, transmission electron microscopy can be utilized in place of scanning, which requires dead specimens. These two options of electron microscopy provides a wider range of success in observation than light microscopy. [bsci223 lecture: you used phase contrast microscopy to observe a wet mount of a soil sample (the first picture above) and a. "goo" sample (the second image above) from the elvis.

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