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Lecture 1

BSCI 223 Lecture Notes - Lecture 1: Gram-Negative Bacteria, Microorganism, Microbiological Culture


Department
Biological Sciences Program
Course Code
BSCI 223
Professor
mciver
Lecture
1

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PAK1.2A
How would go about isolating your pure culture?
By taking the mixed culture, streak plating it with a loop,
leaving it overnight in an environment with a desired growth
temperature for bacteria, one will find different colonies that
formed on the media. Differentiate between the different
clonal populations and streak each one on different plates.
Different colonies represent different cultures. [lab]
PAK1.2b
If your goal is to characterize the ET polyurethane-
degrading microbe (ETPUM), whose results are more
informative: yours or your boss s? Why? What do your
results indicate about the nature of this microbe? Does
its biochemical composition most closely resemble that
of a prokaryote or a eukaryote? Gram-positive or Gram-
negative? Do you agree with your boss conclusion that
the ETPUM is a prokaryotic-eukaryotic hybrid? Why or
why not?
My results are more informative because, unlike my boss, I
isolated my mixed sample into pure cultures. Of course he
would get confusing results because he tested a sample with
different cultures with different characteristics. My results
indicated that the microbe is prokaryotic based on its 70s
ribosomes, circular instead of linear DNA, etc. The bacteria
is gram-negative because of its lack of Leipoteichoic Acid
(LTA) and presence of LPS. The ETPUM is not a hybrid. My
boss made the mistake of regretting to test a pure culture.
His results are a mix of several cultures. [BSCI223 lecture]
PAK1.2C
Come up with at least 2 possible alternative
explanations for the amazing redistribution of the
ETPUM on the Lycra-sealed slide. Both of your
explanations should consider how the microbes sensed
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