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Lecture 4

BSCI 283 Lecture Notes - Lecture 4: Atp Synthase, Signal Transduction, Listeria Monocytogenes

Biological Sciences Program
Course Code
BSCI 283
Daniel Stein

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Metabolism 2/13/17
Flagellar Orientations
o Amphitrichous-flagella at both ends
o Monotrichous-only one flagella
o Peritrichous-multiple flagella
Gliding Motility
o Requires surfactant
o No visible external apparatus
o Different species may use different mechanisms
o Cell body moves relative to adhesion sites
o Proteins form focal adhesion complexes
Movement by Actin Polymerization
o Intracellular pathogens move inside of host cells
o Ex. Shigella dysenteria, listeria monocytogenes
o Polymerizing strand of actin pushes bacteria
o Act A is protein produced by Listeria Stimulates actin polymerization on one
polestick pushes cell
o Swimming by peritrichous bacteria
o Run and tumble
o Random movement, tumble to change direction
o Metabolism produces NADHproton gradientenergy to power motor
o Electron transport chain can generate PMF
o 9 protons per electron pairNADH
o oxygen is terminal electron acceptor
o 3ATP per NADH
o ATP synthase can reverse: hydrolyze ATPpump protons
o Taxis-directed movement
o Aerotaxis-cells attracted to oxygen
o Chemotaxis-response to chemical gradients
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