BSCI 353 Lecture Notes - Lecture 6: Squid Giant Synapse, Squid Giant Axon, Neuromuscular Junction

• School: University of Maryland
• Department: Biological Sciences Program
• Course: BSCI 353
• Professor: kanold

Document Summary

Inject pulse of ca from electrode (no stimulation) 2: still get psp ca in flux is sufficient for nt release, exp 3. 1: results: removing pre ca prevented post response, conclusion: presynaptic ca entry is necessary and sufficient for. Allow for amplification of signal: diverse, like ionotrophic, receptor physiology, what happens when nt binds, experiment: to determine the effect of ach binding to ach receptor, method: patch clamp recordings from postsynaptic membrane that includes ach receptors. Puff on ach record currents: results: observe ach gated ion channels, part 2, pull patches with varying numbers of ach receptors, observe single channel conductance of 2pa iii. Inward current: conclusion: when ach binds to ach receptor, observe transient single channel conductance. Channel cannot function w/o nt: part 3, switch from voltage clamp to current clamp, measure postsynaptic potential. Inhibitor channel permeable to cl- c: reversal potential of ps provides clue to which ion e. f.