BIL 250 Lecture Notes - Lecture 9: Restriction Fragment Length Polymorphism, Dna Profiling, Restriction Site

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Why is 1% of our dna different: crossing over, random alignment, dna replication errors and mutations. Where a marker occurs may or may not be important with regard to function: some markers are neutral and some are not neutral. If you have to lock someone up based on a dna test, would you want to have all of your markers on chromosome 16 or spread out. Types of markers: minisatellite, microsatellites, rflps (restriction fragment length polymorphisms, allele specific probes and primers. Microsatellites are great because: polymorphic, single locus, common in most organisms, neutral. Rare alleles give you very convincing statistical results. Cut the dna with restriction enzymes and look at the pattern. Make that map, cut dna into lots of different pieces and put the puzzle back together. The advantage is that it came earlier. We are using recombination frequencies with traits as the markers. It is not just map, it is 24 maps.

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