BMB 401 Lecture Notes - Lecture 5: Oxaloacetic Acid, Enzyme Inhibitor, Insulin
Document Summary
Pyruvate + coa + nad+ acetyl-coa + co2 + nadh. This is the overall reaction catalyzed by pdc (pyruvate dehydrogenase complex) Step 1: e1 decarboxylates pyruvate and transfers the acetyl group onto its cofactor tpp and in the process co2 is eliminated. Step 2: e2 takes acetyl group from tpp and transfers it onto its cofactor lipoamide. Step 3: e2 transfers acetyl group from lipoamide to coa resulting in acetyl coa! In the process lipoamide is reduced to dihydrolipoamide e2 and the next 2 steps are done to regenerate lipoamide e2. Step 4: e3(ox)fad gets reduced and the disulfide bridge now becomes 2 thiol groups and does this to oxidize the dihydrolipoamide e2. It basically takes 2 h from dihydrolipoamidee2 and puts it on its disulfide bridge reducing it and oxidizing it back to lipoamide. Lipoamide e2 is regenerated but now we must regenerate the enzyme!