CHEM 106 Lecture Notes - Lecture 8: Plasmid, Recombinant Dna, Microbiology
15 Feb 2018
School
Department
Course
Professor
Document Summary
Biotechnology: the use of microorganism, cells, or cell components to make a product. Recombinant dna (rdna) technology is a specific type of biotechnology: insertion or modification of genes to produce desired proteins. We can insert them into yeast for example. After we insert the gene of interest, the bacteria goes along and just starts expressing proteins. It will express the protein you want once it starts transcription/translation, it will start making that protein like it was already there. Insulin, hepatitis vaccine, doesn"t always have to be pharmaceutical. Mutation: use of mutagens (such as chemicals/radiation) to cause mutations that might result in a microbe with a desirable trait. We can actually create artificial selection to get desired traits. Selection: selectively culture a microbe that produces a desired product. A restriction enzyme is an enzyme that cuts specific sequences of dna. Maybe one looks directly for ccga and at that spot it cuts.
