BIOL 1081 Lecture Notes - Lecture 30: Dna Mismatch Repair, Dna Repair, Telomerase
Document Summary
Dna replication & pcr reading: sections 16. 2 & pgs. Although the possible replication mechanisms were relatively easy to deduce, proving which occurs in vivo was a more difficult task. Meselson and franklin stahl used the newly developed techniques of density-gradient centrifugation, to show that dna replication proceeds in a semiconservative fashion: compare and contrast dna replication in prokaryotes and eukaryotes. There is only 1 origin of replication per dna molecule. Origin of replication is formed of about 100- Replication of dna occurs at one point in each prokaryotic dna molecule. Only 2 replication forks are formed in each replicating prokaryotic chromosome, as dna is bidirectional. One replication bubble is formed during dna replication. Initiation of dna replication in prokaryotes is carried out by protein dnaa and dnab. Okazaki fragments are large (1000-2000 nucleotides long) Many origins of replication (over 1000) in each eukaryotic chromosome. Each orgin of replication is formed of about.
