BIOL 2081C Lecture Notes - Lecture 15: Okazaki Fragments, Chromatography, Dideoxynucleotide
Document Summary
The classical experiments performed by meselson and stahl demonstrated for the first time that dna replication proceeded in a semi-conservative fashion, where each strand of the. Dna duplex serves as a template for a new daughter strand. You have learned the fundamentals of dna replication in introductory biology, so we will discuss only the highlights of replication and focus on the application of dna replication to terminator sequencing (sanger sequencing) here. Learning objectives: explain how the chemistry of phosphodiester bond formation between an incoming nucleotide and the growing dna strand dictates that dna replication must occur in the. Hint: here is a helpful mnemonic to remember the directionality of. Visualize the 5" prime end of the incoming nucleotide on the left and the 3" end on the right. It has no oh group which makes the synthesis from further occurring. Fluorescence-labeling: each ddnucleotide for each base pair is labeled and can be seen in bands after electrophoresis.
