BIO 110 Lecture Notes - Lecture 19: Post-Translational Modification, P53, Cyclin-Dependent Kinase 2
Document Summary
Cell cycle control is an ancient feature of eukaryotes. How can we study such genes? (mutate genes and impair function result is lethality) Might kill essential gene and kill organism. Disable protein if temperature is increased slightly. Grow the same mutant strains at low (permissive) and normal higher (restrictive) temperature. Cells with growth defects at low temperatures, but not high temperature must have temperature sensitive mutation in growth" genes. Results: many genes contribute to control of cell cycle. Conclusion: several distinct genes result in cell cycle arrest. Arrest occurs at different stages in the cell cycle. A search for temperature sensitive mutants with cell cycle defects uncovered a number of genes have effects throughout the cell cycle (cdc=cell division cycle) Cell cycle lesson from frogs: oocytes arrest at g2 until stimulated by progesterone. Transfer of cytoplasm from a progesterone stimulated oocyte into cell cycle.
