BIO 110 Lecture Notes - Lecture 10: Cell Fractionation, The Blot, Cell Membrane
3 Aug 2018
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Document Summary
Use of western blots and cell fractionation antibodies can also be used to determine the location of a protein in cell fractionation experiments. One can then disrupt the membranes and protein:protein interactions in each fraction by adding a mild detergent, separate the proteins by gel electrophoresis, and finally conduct a western blot. Experiment to locate a specific cellular protein by cell fractionation can be conducted as follows. Cells are grown on a glass slide (or a piece of tissue is excised from an animal). The cells are homogenized, and the various structures within a cell are separated by centrifugation. The solid support containing the bound protein is called a. The western blot is immersed first in a solution containing the rabbit antibody, and then unbound antibody is washed away. Quaternary structure - some proteins contain 2 or more polypeptide chains that are called subunits of the protein. In an endergonic reaction, energy is absorbed from the surroundings.
