BIS 104 Lecture Notes - Lecture 2: Cytosol, Lysosome, Fragment Antigen-Binding
27 Jan 2018
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Proteins, lipids, sugar: protein encoded by genes. Any cells might have 20,000 different types of proteins. Study one protein or complex or network. Break cells to see what happens genetics: essential genes. Redundancy either of 2 genes are enough: genetics tells use what is necessary, but not sufficient. Put parts back together biochemistry: biochemistry can tell us what is sufficient. Antibodies specifically recognize single proteins: igg 4 polypeptides (2 identical heavy chain and 2 identical light chain) Western blot: is protein is there or not, how much protein (concentration, how big is the protein. Lyse cell boil cell in sds. Unfold protein and coats with sulfate groups (negative charged) Sds page: little = fast, big = slow. Transfer protein to a membrane nitrocellulose. Immunoprecipitation (ip: method to isolate / purify protein or complex or network from the cell, need antibody against protein of interest, way to isolate antibody : antigen complex. Lyse cell shear force dounce homogenizer.
