BIS 104 Lecture Notes - Lecture 4: Antiporter, Calcium In Biology, Alpha Helix
27 Jan 2018
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Dounce homogenizer shear force breaks open cell membrane, keeps organelle membrane in tact. Denatures proteins: non ionic triton x 100. Trypsin enzyme that chops up solubilize protein that is outside of the cell membrane. Membranes impermeable to ions, organic molecules, but membrane allows hydrophobic, small polar, water can cross membrane slowly. Cells with highly charged negative molecules can cross. High levels of negative organic and na+ Animal cells active atp pup out na+ Plant cell solid cell wall prevent burst. Protozoan concentrate water and secrete it. Open/close voltage, ligand and mechanical gate. Change shape when it opens or closes. Slower and doesn"t need energy (down gradient: against diffusion. Burns atp adt +pi to move 2k in and 3 na out. Symporters: one goes down concentration gradient, one goes up concentration gradient. Antiporter: one goes down concentration gradient, one goes up concentration gradient. Treatment drink water + glucose + na = gatorade.
