MIC 102 Lecture Notes - Lecture 17: Bacteriophage Ms2, Sanger Sequencing, Dna Sequencing

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Genome = all of an organisms dna. Genomics = studying the entire genome of an organism. Studying a genome means starting with sequencing that genome. The first gene sequened was the bacteriophage ms2 coat protein gene in 1972. Sanger-style: long reads but slow process and not always accurate. Each plasmid has to be sequenced separately. Genome annotation predicting what features are in the sequence: gene identification, operons, promoter sequence. Compare predicted gene sequence against database of known genes. If genomics is the study of an entire genome in total, metagenomics is the study of many entire genomes in total. Genomics and metagenomics can be used to study organisms that we cant grow in the lab. Traditional isolation techniques might only pull out 0. 05% of bacteria from an environment sample. Can study a mixed sample from any environment source. Early 16s rrna sequencing was the first of this type of research that has grown to encompass studying whole genomes.

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