BIO SCI 194S Lecture Notes - Lecture 2: Biosafety Cabinet, Standard Operating Procedure, Biosafety
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(LEC. 2) UCLC Lab Safety
• What is the minimum wavelength level needed for penetration into the dermis?
• What four items listed below are considered Administrative controls in laboratory safety?
- Chemical hygiene plans
- Required training
- Policies and Standard Operating Procedures (SOP)
- Signs and Labels
• According to the article what are the downsides of using UV light in a biosafety cabinet?
- Areas hidden from the light are not disinfected
• What is the optimal UV wavelength range for disinfection of biosafety cabinets?
- UVC with wavelength between 290 and 20 nm
• How has UV light been used in research labs?
- As an effective way to disinfect bio safety cabinets
• According to the CDC and NIH in the article, what are the two risks associated with over
exposure to UV light to humans
- Corneal burn
- Skin cancer
• CJ Standard Operating Procedure is the written safety and health measures for hazardous
• CJ Chemical Hygiene Plan provides prudent practices and procedures for use of chemicals
in the laboratory.
• What are the advantages of UV light in laboratories
- It is an effective germicide and virucide
- UV light leaves no residue in comparison to other disinfectants, the
disinfecting actions stops upon de-energizing the bulb.
- It helps to prevent cross contamination of PCR samples.
• CJ Lab Safety Manual is the document that addresses all the hazards in the laboratory
• According to the article, what two steps should you take to protect yourself against UV
- Do not leave the UV light on over night, turn it off when working with the
- Wear Personal Protective Equipment such as safety glasses, latex gloves and
your lab coat
• CJ USE AUTHORIZATION gives authorization to work with certain hazardous materials or