BIO SCI 93 Lecture Notes - Lecture 17: Cloning Vector, Recombinant Dna, Dna Ligase

• School: UC-Irvine
• Department: Biological Sciences
• Course: BIO SCI 93
• Professor: Diane O' Dowd

Document Summary

Reading guide for lesson 17 (pages 412 417, 432 433) Complete this reading guide as you read the textbook pages listed above. You might not have to read every word on every page, rather pay close attention to the questions in this guide and answer them as you work through the textbook. Also pay close attention to the terms that are underlined: these are key terms that you should know the definitions of and be able to apply in new situations. Start on page 412 (making multiple copies of a gene or other dna segment) Using these terms, the text, and figure 20. 5, explain how a research can clone a gene using bacteria. Explain how restriction enzymes encounter restriction sites to produce restriction fragments. Explain how researchers use restriction enzymes and dna ligase to create recombinant dna plasmids. Answer the draw it question for figure 20. 6 on page 413 in the space below.