BIO SCI 99 Lecture 9: Bio 99 Lec 9
12 Sep 2018
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Int: initiator sequence: tata box, phosphorylation of ct domain during initiation, dephosphorylation after elongation, pol i: rrna, ubf: upstream binding factor. Sl1: protein complex that includes the tata-binding protein: pol iii: trna, 5s rrna, promoter sequences within the coding sequences, major groove = more. Yes - gst-foxd3 and oct-4 form a band - presence indicates that it is interacting: what is the purpose of using gst alone, oct 4 does not bind to gst. Homework questions: you decide to perform a chromatin immunoprecipitation to see whether your favorite gene, the cool gene, is associated with h3 histones that are methylated at lysine 9 (on the histone tail). You then want to determine whether this methylation also means that expression of the. Cool gene is inhibited in cells relative to cells where the chromatin at the cool gene is not methylated. Select all that apply: some genes have promoters that are found within the open reading frame.
